Unison mode can use 1 to 5 voices. If it’s a simple mono 1 voice patch, switching unison off should make it poly without changing the sound much. If it’s using 2-5 voices or Chord mode, then the sound will change as you’ll have one voice per note instead of 2-5.
Some ways around this: try using effects to thicken up the sound, like a short delay or chorus, or multitrack the unison patch in a DAW or sampler to get “polyphony” from it. Or just live with the thinner poly sound, which will work better than a thick unison sound when playing chords from it anyway.